Objective: To explore and summarize the model of ischemic reperfusion in Langendorff perfused heart. Methods:Twenty six Wistar adult rats were divided into two groups randomly: control group and model group, 13 rats in each group. Each rat undergone open-chest surgery, the hearts were removed and immediately canulated and effectively perfused in Langendorff apparatus. Control group hearts only received stabilized perfusion for 105 min and had no ischemia. All the hearts in model group were stabilized for 20 min and then received 30 min ischemia by ligationing LAD（ Left anterior descending coronary artery ）and 75 min reperfusion。 Evans blue, TTC staining to observe the myocardial infarction area. Compare the level of perfusate CK and LDH ，hemodynamic index , heart rates in various groups at different time：end of 20 min stabilized perfusion, end of 30 min Ischemia，Reperfusion 15 min，30 min and 75 min. Results：According to the Evans blue, TTC staining, myocardial enzymology and hemodynamic indexes, the model group had significant difference compared with the control group. Conclusions: Rat model of ischemic reperfusion in Langendorff perfused heart is stable and effective. This model provides a new way to research on ischemia-reperfusion injury.