Objective To detect in the supernatant of superoxide dismutase SOD activity of SH-SY5Y cells, learn about caloric restriction under culture conditions, the cells’ resistance to oxidation. Methods SH-SY5Y cells cultured in vitro, divided into six groups: control(3.0g/L-glucose) group, H2O2(250umol/L)group, low glucose(2.0g/L-glucose) group, low glucose H2O2group, resveratrol(1.25、5umol/L) group, resveratrol(1.25umol/L) H2O2 (250umol/L) group. Determination of superoxide dismutase activity of groups of cells. Results Under conditions of caloric restriction, determination of superoxide dismutase activity: Low glucose and resveratrol(1.25umol/L) pretreat cells for 24 hours, 250umol/L H2O2 treat cell a hour, superoxide dismutase activity showed that, compared with control group, H2O2 group decreased, low glucose group has a significant increase, resveratrol(1.25umol/L) group has no significant change; Compared with H2O2 group, low glucose H2O2 group increased significantly, slight increase in resveratrol H2O2 group. 250umol/L H2O2 treat cell 7 hours, compared with control group, H2O2 group decreased, low glucose group has a significant increase, resveratrol(1.25umol/L) group has no significant change; Compared with H2O2 group, low glucose H2O2 group increased significantly, slight increase in resveratrol H2O2 group. Low glucose H2O2 group significantly higher superoxide dismutase activity of resveratrol H2O2 group. Conclusion Low sugar training method can improve activity of superoxide dismutase in cell culture media, suggesting that caloric restriction by increasing the antioxidant enzymes activity in SH-SY5Y cells, reduce levels of oxidation products, reduce the level of oxidative stress, protects cells from oxidative damage attacks and slow the occurrence and development of related diseases. Resveratrol can’t improve activity of superoxide dismutase in cell culture media against oxidative damage.