Objective Mamu-B*007:03-sv1 is a splice variant of major histocompatibility complex class I in Chinese rhesus macaque devoid of α3 domain. In this experiment the basic information about expression and cellular location of Mamu-B*007:03-sv1 was got and compared with the full-length Mamu-B*007:03 genes. Methods Eukaryotic expression vectors of Mamu-B*007:03-sv1-myc-pEGFPN3 and Mamu-B*007:03-myc-pEGFPN3 were constructed and transfected to 293T cells. The expressions of the two genes in cells were detected by Western blotting methods, and the cellular locations of the two genes were analyzed by immunofluorescence and laser confocal microscopy. Results Mamu-B*007:03-sv1 and Mamu-B*007:03 were all expressed in the 293T cell and were all glycosylation. While Mamu-B*007:03 gene was expressed on the cell membrane, and Mamu-B*007:03-sv1 gene was expressed in both intra-cellular and cell membrane. Conclusion The expression and cellular localization of Mamu-B*007:03-sv1 deviod of α3 was significantly different from the full length Mamu-B*007:03 gene. Further researches about functions of Mamu-B*007:03-sv1 need to be done.