Objective Atfu-B*sv1 with α2 domain deletion is a splice variant of major histocompatibility complex class I B in Ateles fusciceps. In this experiment the basic information about expression and cellular location of Atfu-B*sv1 was got, and aimed at compared with the full-length Atfu-B genes, observed the different from them. Methods Eukaryotic expression vectors of Atfu-B*sv1、 Atfu B*02:03 and Atfu-B*03:01 were constructed and transfected to 293T cells. The expressions of the three genes in cells were detected by Western blotting methods, and the cellular locations of the three genes were analyzed by immunofluorescence and laser confocal microscopy. Results Atfu-B*sv1、Atfu-B*02:03 and Atfu-B*03:01 were all expressed in the 293T cell and were glycosylation . All the three genes were expressed on the cell membrane. Conclusion The expression and cellular localization of Atfu-B*sv1 was not significantly different from the full length Atfu-B genes. Further researches about functions of Atfu-B *sv1 need to be done.