Objective: fMRI animal experiments that require sustained anesthesia to provide a simple, convenient, and safe manipulation condition. This investigation was designed to confirm the appropriate anesthetic regimen for fMRI experiment of rhesus. Method: Seven rhesus from Academy of Military Medical Sciences are female, weight 4-7 kg using continuous intravenous anesthesia (ketamine propofol) . Ketamine 10mg/kg im until the rhesus sleep then establish a continuous intravenous access. continuous injected ketamine100mg/20ml, and propofol 3mg / kg / min, then intermittent ketamine 1mg/kg/30min. The rhesus placed in a special experiment rack and the incisor hold up to keep the airway open. During the whole test we monitored breathing, heart rate, corneal reflex and pupil. Results: 44 times experiments successfully completed, no animal restlessness, respiratory depression, arrhythmia, deaths and other serious complications. At the end of the experiment, the experimental animals awake soon. Conclusion: continuous intravenous anesthetic of propofol and ketamine could provide more secure, convenient and practical method for fMRI animal experiments.