Objective Use the special and double immunohistochemistry staining method to obsearve the typical histopathological change in experimental acaques diabetes model.Methods we used STZ (30 mg/kg of body weight, intravenously, 3days)to five macaques. The animal was sacrificed when impending death. The histopathological variety of pancreas,heart,kidney and spleen were observed with H.E,PAS,Masson,Sirius red,toluidine blue and double immunohistochemistry staining. ResultsThe quantity of pancreatic island decreaseded obviously,the reminders showed atrophy. fiber hyperplasia were observd in exocrine pancreaswith masson stain. In kidney, There were focal chronic nephritis, renal interstitial fibre hyperplasia, parts of glomerular and renal tubular atrophy.Small arteria in spleen was thickening with PAS stain. In double immunohistochemistry staining, the quantity of brown-colored A cell is increased while the pink-colored B cell is decreased. Concusion The use of H.E combined with special and double immunohistochemistry staining is a better method to evaluate the Diabetes Mellitus model.