Abstract: Objective To explore the effect of Adriamycin with different doses used for the Focal Segmental Glomurular Sclerosis Nephrosis rat model . Methods The Focal Segmental Glomurular Sclerosis Nephrosis rat induced by left nephrectomy plus intravenous injection with different Adriamycin doses,to observe the effect of different Adriamycin doses on rat model of survival rate,24-hour urinary protein excretion, serum creatinine,urea nitrogen, and kidney pathology.Results the survival rate decreased with the increase of injection dosage in different doses group and survival rate of injection dosage of 4.5mg/kg~6mg/kg was reduced to less than 50% at fourth week and eighth week,survival rate had a significant negative correlation with injection dosage at eighth week（r=0.9045，P<0.01）. Each group of injection dosage appeared urinary protein significantly increased at first week（P<0.01）, serum creatinine significantly increased at second week（P<0.01）and urea nitrogen significantly increased at fourth week（P<0.01）, and all showed progressive increase, serum urea nitrogen of each group is higher than the normal except the 3mg/kg group（P<0.01，P<0.05）, 24-hour urinary protein excretion, serum creatinine and urea nitrogen had a significant positive correlation with injection dosage at eighth week（r=0.9429，P<0.01 ；r=0.9384，P<0.01；r=0.9568，P<0.01）. Renal pathology of all groups showed Focal Segmental Glomurular Sclerosis,and the hardening degree aggravation while injection dosage rising.Conclusion Mortality of 3mg/kg group is Lowest and accord with Human Focal Segmental Glomurular Sclerosis，it is the more ideal dose of Adriamycin rat model.