Objective To observe the change of Neuroendocrine hormone and Electrolyte levels in WHBE Rabbit with Irritable Bowel Syndrome, to explore the regulation role of nerve-humoral-endocrine system. Methods The irrtable bowel syndrome rabbits model was made by using the methods of moist heat, stress and stimulation adding with low-dose induced cathartic induced. When modeling 9d and 14d, take blood samples, testing the changes of rabbits serum levels of dopamine (DA), 5-hydroxytryptamine (5-HT), adrenocorticotropic hormone (ACTH), cortisol (COR), β-endorphin (β-EP) hormone and K , Na , Cl- content, and compared with JW rabbit. Results Compared with the normal control group, model 9 days group WHBE rabbits DA, 5-HT, ACTH, β-EP and COR significantly increased (P<0.05, P<0.01), Na , K content were significant with lower (P<0.01), while the JW rabbits ACTH, β-EP and cortisol are significant with increased (P<0.05, P<0.01), K content significantly with lower (P<0.05); with the normal control group ratio, model 14 days group WHBE rabbit 5-HT, ACTH, β-EP were significantly increased (P<0.05, P<0.01), JW rabbits ACTH significantly increased (P<0.05). The correlation analysis showed, IBS model group serum 5-HT, COR and Na , K concentration showed a significant negative correlation (P<0.05, P<0.01), serum DA, β-EP level and Na , K , Cl-concentration was a significant negative correlation (P<0.05, P<0.01), ACTH levels with K concentration was significantly negatively correlated (P<0.01). Conclusion IBS is not only associated with elevated HPA axis excitability, but also humoral factors, nerve-humoral- endocrine system is involved in the occurrence of WHBE rabbit with IBS.