Objective To measure IL-1β, IL-1R1, IL-8, IL-8R and IL-15 genes expression in rabbit sacculus rotundus using the Liquid suspension array system, and evaluate the method. Methods The simultaneous measurement and quantity of multiple genes mRNA expression in rabbit sacculus rotundus was detected by the Panomics QuantiGene Plex 2.0 Assay of Affymetrix using the bDNA signal amplification and beads analysis techniques. Then, this method was applied in the detection of rabbit interleukin gene. Results Content of IL-1β, IL-1R1, IL-8, IL-8RA and IL-15 gene can be detected simultaneously. Gene expression of IL-15 in WHBE rabbit is significantly higher than JW rabbit (P<0.05), and gene expression of IL-1R1 in WHBE rabbit is significantly higher than JW rabbit (P<0.01). Also, the relative expression quantity of IL-8RA gene in WHBE rabbit is higher than JW rabbit (P<0.05). Conclusion Liquid suspension array method for detecting rabbit interleukin gene was established. Gene expressions of IL-15, IL-1R1 and IL-8RA in WHBE rabbit were higher, and all the results reveal WHBE rabbit may have unique immunological properties.