Objective We examined the inclusion ratio of SMN2 exon 7 in different tissues from SMA mice and screened related splicing factors. Methods Ratios of SMN2 exon 7 inclusion in neural and non-neural tissues were analyzed by RT-PCR and non-denaturing PAGE electrophoresis. The expression levels of splicing factors in the brain, spinal cord, and non-neural tissues were analyzed by QPCR. Results The inclusion ratio of SMN2 exon 7 in brain and spinal cord tissues was significantly higher than that in non-neural tissues. The QPCR screening result showed that the expression of Nova1 / 2 was significantly higher in the brain and spinal cord than in most non-neural tissues. The difference analysis showed that the mRNA levels of Srsf7 / 9 and Nova2 in the spinal cord of SMA type I mice were significantly higher than those in control mice. Conclusions The high expression of Srsf7 / 9 and Nova1 / 2 is positively correlated with significant increases in the inclusion ratio of SMN2 exon 7 in neural tissues, indicating that Srsf7 / 9 and Nova1 / 2 may be involved in the splicing regulation of SMN2.