Hemophilia is a monogenic hereditary disease whose pathogenesis includes the reduction of coagulationfactors caused by mutations in genes encoding coagulation factors. The clinical manifestation of this disease is recurrentbleeding, which can be life-threatening in severe cases and cannot be cured. Currently, gene therapy might have thepotential to cure hemophilia. In gene therapy research, animal models are commonly used. The mouse hemophilia model isuseful because of its convenience for transportation and lower research costs when compared with other large animal models.It has become a hot spot for research using animal hemophilia models. After more than 20 years of development, technologyto generate a mouse hemophilia model has gradually matured. The aim of this paper is to summarize the research progress in generation of a mouse hemophilia model.