Objective To establish a rapid genotyping method for detection of Pasteurella pneumotropica as astandard revision and effective control, in view of problems with classification of P. pneumotropica and its high infection ratein laboratory animals. Methods Genetic polymorphism and molecular epidemiological analyses of 314 isolates and twostandard strains of P. pneumotropica from laboratory animals were performed using an amplified fragment lengthpolymorphism ( AFLP) method. Results The AFLP method divided tested isolates into 11 gene clusters and 190genotypes; gene cluster AC7 was the main epidemic group. Polymorphic bands mainly occurred between 31~36, and theSimpson diversity index was 0. 992. Conclusions This study shows that P. pneumotropica genotypes are abundant inlaboratory animals in Beijing area, and different strains represented by the two standard strains have significantly differentpolymorphisms. Our results indicate the characteristics of cross contamination, abundant sources, and long-term contamination in some laboratory animals of different sources.