Objective To construct a nude mouse model of granulocytic leukemia with M1 cells. Methods Ninefemale BALB/ c nude mice, 5-6 weeks old, were randomly divided into three groups (n=3 per group): group A mice werethe blank control group, and groups B and C were inoculated via the tail vein with a logarithmic growth phase M1 cellsuspension (1×10⁶ and 5×10⁶, respectively). The general condition of the mice was observed. Blood samples, peripheralblood leukocyte differential count, and peripheral blood CD33CD117 positive cells were detected on days 0, 10, 20 and 30after modeling. On the day 30 or at the time of death, tissue sections were taken for pathological examination. Results Themice in the model group developed symptoms such as wilting and dorsiflexion on days 10-15 after inoculation. Comparedwith the blank group, the number of peripheral blood leukocytes in each mouse injected with 8 × 10⁶ M1 cells wassignificantly increased on the 30th day ( P <0. 05). On the 30th day, the proportion of peripheral blood leukemia cells inthe model group was significantly higher than that in the blank group ( P <0. 05), Compared with the blank group, theproportion of CD33+ CD117+ cells in the bone marrow of the other groups increased, and the high dose group was the mostsignificant. ( P <0. 05), and that on day 30, leukemia cells infiltrated into the spleen of model mice. Conclusions Theinjection of 8×10⁶ mouse leukemia M1 cells into the tail vein of BALB/ c nude mice can induce an acute myeloid leukemia model consistent with the biological characteristics of acute myeloid leukemia.