Objective To investigate the prevalence of bovine coronavirus (BCV) in bovine herds and bovinederivedbioproducts. Methods A fluorescent quantitative PCR (FQ-PCR) method for BCV was developed based on a pairof primers and a TaqMan probe, in accordance with the published sequence of BCV. Results The assay could specificallydetect BCV and had good sensitivity, with a limit of detection of 40 copies. The FQ-PCR method achieved a good linearrelationship within the template concentration range from 10³to 10⁷copies/ μL, with a correlation of 0. 999. Theamplification efficiency of the assay was 96. 195%. A total of 64 bovine rectal swabs and 33 bovine-derived bioproducts weresubjected to the FQ-PCR assay, and the positivity rates were 1. 5% and 6%, respectively. The positive sample wasamplified with another pair of primers for the N gene. Conclusions The results of this study demonstrated that the PCRproduct had 99% similarity to BCV, suggesting the existence of a BCV epidemic in bovine herds and the potential risk of BCV contamination in bovine-derived bioproducts.