Objective To provide a simple, convenient, and safe anesthesia method for long?term operations in macaques. Methods Ten male Macaca mulatta macaques were anesthetized by a combination of intravenous anesthesia with propofol and isoflurane inhalation. The induction time, duration of anesthesia, and recovery time were recorded. After continuous monitoring of blood oxygen saturation, respiratory rate, and heart rate, the feasibility and effectiveness of the anesthetic scheme was evaluated by data analysis. Results All 10 monkeys survived the experimental anesthesia protocol. The anesthesia induction time was short and the onset was rapid. During anesthesia, vital signs were stable, and the sedation, analgesia, and muscle relaxation were satisfactory. Conclusions Combined anesthesia induced by propofol and isoflurane is a safe and effective method for long?term anesthesia of macaques.