Objective To provide a simple, convenient, and safe anesthesia method for the establishment of a M1 segment of middle cerebral artery occlusion model in rhesus monkey or other large laboratory animals. Method Twenty male rhesus monkeys weighing 7-11 kg (ages 7-9 years) from Academy of Military Medical Sciences were used in this study. Sumianxin injection combined with 0.1 mg/kg ketamine was given before endotracheal intubation (ID:4.5-5.5#). The animals were then transported to an interventional operation room, where the intravenous access was established and a urinary catheter was inserted into the urinary bladder. Mechanical ventilation was used during the surgery, propofol was continuously injected in a speed of 2-4 mg/kg/h, and Sumianxin-ketamine could be given if necessary to maintain adequate anesthesia depth. The dose was adjusted according to vital signs of the rhesus such as body movements, physiological parameters, and demand of surgery. Brain MRI examination was performed before and after thrombolysis. Anesthetic injection was suspended and the animals were allowed to have a spontaneous breathing every time before the MRI text. Heart rates, temperature, non-invasive blood pressure, and SpO₂ were monitored during the whole surgery. Blood samples were taken from the radial artery for blood gas analysis after anesthesia induction and during operation. Results All the 20 animals underwent the operation successfully, no animal had restlessness, respiratory depression, arrhythmia and other serious complications. At the end of the study, animals awake soon. Fifteen of them survived longer than 24 hours, only 5 died from serious cerebral hemorrhage and larger cerebral infarction. Conclusions General endotracheal anesthesia is safe for rhesus monkeys during such interventional operation and MRI examination.