Objective To study the effect of heterozygous deficiency of SGT gene on the hematological and biochemical parameters of mice in young and elderly ages.Methods Blood samples were analyzed for complete hematological and biochemical parameters from heterozygous SGT-deficient and wild-type mice of 10-weeks and 6-months old mice, respectively. Results Age-related changes in most indexes were found statistically significantly different between young and elderly mice with the same genotype. Compared with the wild type at the same age, the platelet large cell ratio (P-LCR) was lower in young heterozygous SGT-deficient mice. However, platelet count, plateletcrit (PCT) and neutrophil count were more significantly lower in elderly heterozygous SGT-deficient mice (P<0.05). There was no significant difference for biochemical parameters ALT, AST, LDH, urea nitrogen, creatinine and blood glucose. Total and unconjugated bilirubin as well as ALP were significantly higher in elderly heterozygous SGT-deficient mice but not for conjugated bilirubin (P<0.05). In addition, significant differences existed for the lipids between two elderly groups (P<0.05). Conclusions Heterozygous deficiency of SGT gene induced changes of some hematological and biochemical parameters in elderly mice. It provides helpful information for further investigation on SGT involvement in some biological and pathological processes.