Objective To establish a new orthotopic gastric cancer model in nude mice.Methods The BALB/c nude mice were anesthesized by Matrx VIP 3000 type gas anesthesia systems. Orthotopic gastric cancer models were established by embedding method, thread-ligation method or gastric bursa method in the nude mice. The tumor growth after transplantation in living body was detected by a Caliper IVIS Kinetic small animal imaging system. Samples of the transplanted tumor tissues for histological examination were taken at 28 days after transplantation.Results The orthotopic gastric cancer model was successfully established by embedding method. Compared with the models prepared by other commonly used methods, it showed obvious advantages such as a high success rate of transplantation, simple operation, short time, less difficulty, and less direct contact of the implanted tumor tissue with other organs, etc.Conclusions Large amount of mouse models of orthotopic gastric cancer can be prepared rapidly by the embedding method, thus, provide a convenient tool for related research of gastric cancer in future.