Objective In this study we investigated the analgesic effect of oxysophocarpine (OSC) on carrageenan-induced inflammatory pain in mice and to explore its possible mechanism. Methods Mouse ear swelling test and carrageenan-induced paw edema were used to investigate the effects of OSC (10, 40 and 80 mg/kg) on inflammatory pain in mice. The mechanical hyperalgesia and allodynia were measured by von Frey filaments. Paw edema was assessed by toe volumetric measuring instrument. text the content of TNF-α, IL-1β, IL-6 and PGE₂.Results OSC showed a significant anti-inflammatory effect in the mouse ear swelling test. OSC (10, 40 and 80 mg/kg) also significantly reduced the paw edema volume and improved mechanical allodynia threshold value on carrageenan-induced inflammatory pain, as well as relieved paw tissues inflammatory damage and reduced the numbers of neutrophils in mice. OSC significantly suppressed over-expression of TNF-α, IL-1β, IL-6 and PGE₂. Conclusions Based on these findings we propose that OSC attenuates inflammatory pain by suppressing the Pro-inflammatory cytokines.