Objective To determine and compare the parameters of blood, heart rate, carotid artery pressure, ventricular pressure and organ indexes of male and female FMMU albino guinea-pig.Methods A total of 30 guinea-pig about 250~350 g weight were fed for a week, then blood physiological and biochemical index, blood gas and carotid artery pressure were determined, ventricular pressure were measured by arterial catheterization and heart rate were measured on the respiratory support condition. Finally,weight of guinea-pig body and main organ were weighed. Result The difference of platelet count, glutamic-pyruvic transaminase,total bilirubin and lactic acid were significant(P<0.05 or P<0.01). The difference of carotid diastolic blood pressure and mean blood pressure, left ventricular systolic pressure and mean pressure were totally significant(P<0.05 or P<0.01). And there were no significant differences in weight and organ coefficient of main organs. Conclusion There were differences in blood physiology and biochemistry, blood gas, carotid artery pressure and ventricular pressure in different gender of the ordinary grade FMMU albino guinea-pig.