Objective To investigate endoscropic assist in rat orotracheal intubation. Methods 40 rats were randomly divided into two groups. The procedure time, total time, intubation frequency, the success rate of the first intubation and the survival rate in 24h were compared between the conventional group and the endoscropic group. Results The total time in two groups were nearly the same. But the procedure time and frequency of intubation were significantly less in the endoscropic group than those in the conventional group. The success rate of the first intubation in the endoscropic group was obviously higher than that of the conventional group. There was no obviously difference between the survival rate after extubation in 24h between groups. Conclusion Endoscropic assist is superior to the conventional method and worth promoting.