Objective To detect the blood parameter, blood biochemical and electrolyte indices of the F1 generation of Rongshui miniature pig (RMP). Methods The blood of 43 female and 42 male RMPs of 4^th month old, and 36 RMPs of 12^th month old (half male and female) were extracted from jugular vein. And the blood parameter, blood biochemical and electrolyte indices were detected by blood analyzer and automatic biochemical analyzer. Results In the same month-old RMP, no significant difference between male and female were found in most indices of blood parameter, blood biochemical and electrolyte indices. On the other hand, many indices were difference between 4^th month old and 12^thmonth old RMPs of same gender. Compared with the 4^th month old RMP, the 12^th month old RMP decreased significantly in WBC and PLT, increased in HGB (P < 0.05) while RBC was the same (P > 0.05). Serum ALT, AST, ALP, CK (male), LDH(male), A/G, BUN, GLU (female), CHOL (male) and K⁺ decreased significantly (P < 0.05), while serum TP, TBIL, CR and Ca²⁺ increased significantly (P < 0.05),but serum CHOL, TG, HDL-C and LDL-C were not different. 86.4%(19/22) biochemical and electrolyte indices in RMP were in/or close to the range of normal value of human. Conclusion Most of the blood parameter, blood biochemical and electrolyte indices of RMP were close to human's normal value.