Objective To provide reference of the general physiological index, blood physiology and biochemistry for middle and old-aged cynomolgus monkey. Methods 119 cynomolgus monkey aged from 11～16 (80 were males and 39 were females) were involved in the study. We detected the general physiological index(body weight, the crown-rump length and waist circumference), hematology and blood biochemistry parameters respectively and compared these parameters between males and females. Results Between males and females, there were significant differences in Body weight, the crown-rump length and Waist circumference of the general physiological index(P < 0.01),NEUT#、LYMPH#、EO#、BASO#、NEUT%、LYMPH%、EO%、BASO%、MCV、MCH、RDW-CV、PCT、MONO%、HGB、HCT、MCHC、RDW-SD、MPV of hematology(P < 0.01 or P < 0.05)and TBIL、ALB、GLO、A/G、ALP、GLU、UREA、CREA、TCH、TG、CK、ALT、GGT of blood biochemistry(P < 0.01 or P < 0.05). There were no significant differences in other parameters(P > 0.05). Conclusion It provides reference of general physiological index, blood physiology and biochemistry parameters of cynomolgus monkey and basis for their application in biomedical domain.