Objective To investigate the effects of morroniside on the expression of Wnt7a and APC in rat cortex of focal cerebral ischemia/reperfusion. Methods After middle cerebral artery occlusion (MCAO) model was performed SD rats were divided into groups of sham, model, and morroniside (30 mg/kg, 90 mg/kg,270 mg/kg). The expression of Wnt7a and APC were evaluated by Western blot. Results Compared with the sham group, the expression of Wnt7a in rats of MCAO model was increased, and the expression of APC was decreased. Compared with model group, morroniside at the concentrations of 30 mg/kg, 90 mg/kg, 270 mg/kg decreased APC expression respectively, while 270 mg/kg morroniside-treatment obviously increased the expression of Wnt7a. Conclusion Morroniside could increase the expression of Wnt7a, and inhibit the expression of APC after ischemic injury, therefore activating the Wnt signaling pathway.