Objective To provide a good evaluation for research the hyperthyroidism drugs, two kinds of hyperthyroidism animal models were copied, and compared the similarities and differences of their formability and sustainability. Methods Euthyrox-induced model: The rat was taken Euthyrox (50μg·100g^-1 rat weight) for 21 d. And then the blood and urine were collected in 1 d, 7 d and 14 d after last time oral administration of Euthyrox;Yersiniaenterocolitica-induced model: the rats were injected Yersiniaenterocolitica(YE) in 0、5、10、15、20 (IOCV). And then the blood and urine were collected in 5d, 15d and 25d after last IOCV of YE. Results Euthyrox-induced model: T3, T4, FT3, FT4 were increased significantly, but returned normal 1 week later in the model group, PCA showed that model group deviated from the blank group, but recovered 2 weeks later; Yersiniaenterocolitica-induced model: T3 and T4 were increased significantly in 15 and 25 d, TSH significant raised in 25 d, the model group deviated from the blank group in PCA, and didn't return to the blank group in 15d or 25d. Conclusion Euthyrox-induced modelhas good formability but recovered quickly, which is suitable for prevention of drug delivery on efficacy evaluation; Yersiniaenterocolitica-induced model with good formability and sustainability could be used for treatment of drug delivery on efficacy evaluation.