Objective To investigate the influence of Siraitia Grosvenori and Rehmannia Glutinosa on the Hematopoietic stem cells proliferation and function. Methods Cells from the peripheral blood, spleen and bone marrow of mice were stained with indicated antibodies, and analyzed by flow cytometry. Mice were divided 3 groups: control group, Siraitia Grosvenori treatment group and Rehmannia Glutinosa treatment group. After 4.5Gy IR treatment, mice divided 4 groups: control group, 4.5Gy IR treatment and feed with normal food, 4.5Gy IR treatment and feed with Siraitia Grosvenori and 4.5Gy IR treatment and feed with Rehmannia Glutinosa for 1 month. Results Mice fed with Siraitia Grosvenori and Rehmannia Glutinosa decreased the percentage of B cells and increased the percentage of M cell. For HSCs, the number of HSCs was increased, especially the number of LT-HSCs. After 4.5Gy IR treatment, mice fed with Siraitia Grosvenori and Rehmannia Glutinosa increase the number of HSCs, and increased the percentage of M cells. Conclusion Siraitia Grosvenori and Rehmannia Glutinosa promote the hematopoietic stem cells and progenitor cells proliferation and function and recover the damage that caused by IR treatment.