Objective To investigate a antitumor effects of mouse original monoclonal antibody against hMIC-1 as intravenous administration with human pancreatic tumor in vivo and providing experimental data.Methods The fourty-eight mice were randomized into eight groups for loaded with two pancreatic tumor cell lines panc-1 or sw1990 respectively, and individual tumor growth was observed, antitumor efficacy was evaluated after using mouse original monoclonal antibody against hMIC-1 by intravenous administration.The pathological change with formalin fixed, paraffin embedded tissues section was viewed.Results There was a significant difference in tumor volume and weigt in intravenous injection of mouse original monoclonal antibody against hMIC-1 on load pancreatic tumor with nude mice group compared with that in the control group after four week treatment, and the mouse original monoclonal antibody against hMIC-1 demonstrated a close association between inhibition of tumor volume growth and dose-effective in the two xenograft models examined. Under examined microscope, the pancreatic tumor tissue was destroyed evidently in mouse original monoclonal antibody against hMIC-1 group.Conclusions The antitumor effect of intravenous injection for mouse original monoclonal antibody against hMIC-1 is better than that of systemic using gemcitabine.