Objective To study the isolation,culture, adipogenic and osteogenic induction Tupaia bone marrow mesenchymal stem cells(BM-MSCs). Method The BM-MSCs from tupaia were isolated and expended by combination of gradient centrifugation and adherence culture, then subcultured and observed for morphology under inverted phase contrast microscope. BM-MSCs were induced to adipocytes.and osteoblasts in vitro Result Cells were spindle or triangle-shaped,and clone proliferation.Cells were successfully induced into adipocytes.and osteoblasts Conclusions The method of isolation BM-MSCs from tupaia by combination of gradient centrifugation and adherence culture is simple and feasible, BM-MSCs have differentiation potential into adipocytes and osteoblasts.