Objective To observe the expression of insulin-like growth factor 1 (IGF-1),insulin-like growth factor binding protein 3 (IGFBP-3) in rats' serum with non-alcoholic fatty liver disease(NAFLD) and the impact of Polyene phosphatidyl choline. Methods NAFLD model was induced by feeding the SD rats with a high-fat diet, with Polyene phosphatidyl choline to intervene.Observe the pathological changes of the rats' liver tissue dynamically after HE staining.Detect the contents of IGF-1,IGFBP-3 in serum dynamically through the immuno radio metric assay(IRMA). Results There were no obvious exception of the liver tissue pathology in the normal group at each time.With the extension of a high-fat diet,the liver tissue of the model group increased on fatty change,the degree of inflammation,the balloon sample change,the NAFLD activity score at 4,8,12weeks.IGF-1,IGFBP-3 in serum were decreased significantly,and the model set was significantly lower than the normal group at the same phase.After the application of polyene phosphatidyl choline,the degree of rat liver tissue inflammation and the NAFLD activity score were reduced significantly when compared with model group,while the level of IGF-1,IGFBP-3were significantly higher. Conclusion The levels of IGF-1,IGFBP-3 in the rats' serum reduce in the development of non-alcoholic fatty liver disease.