Objective To investigate the role of hypoxia on the expression of DNA methylation and histone acetylation related enzymes.Methods Primary cultured rat astrocytes treated with oxygen-glucose deprivation (OGD) were used. The expressions of DNA methyltransferase (DNMTs), Methl-CpG binding domain protein 2(MBD2), CREB-binding protein (CBP), Histone Deacetylase 3(HDAC3) were examined by Western blot.Results DNMTs(DNMT3A and DNMT1) levels were increased by 49% and 83% compared with control and the level of MBD2 level was decreased by 36% after OGD for 48h. HDAC3 level was up-regulated by 2.1 times after OGD for 48h, however the expression of CBP was dramatically increased by 20 times compared with control.Conclusion The expression of DNA methylation and histone acetylation related enzymes might alter under hypoxia in astrocytes leading to gene transcription.