Objective To select a best mouse strain for establishing a better animal model of alcoholic fatty liver through comparison study. Methods BALB/c,C57 and C3H were choose to establish alcoholic fatty liver model through alcohol gavage and the serum biochemical indicator and the results of pathology were compared.Results Weight gain and liver index in BALB/c model group were lower than those of the control group(P < 0.01,P < 0.05);weight gain in C57 and C3H model group were lower than that of the control group(P < 0.05,P < 0.01). Compared with the control group,ALT,AST levels in BALB/c model group were markedly increased(P < 0.01);ALT、AST、TG levels in C57 model group were increased(P<0.01);liver cell cytoplasm appeared a large number of scattered fat droplets in BALB/c mice model group,and fat droplets in C57 model group were more than those of BALB/c and C3H model group,the hepatic steatosis degrees in C57 model group were obviously serious.Conclusion C57 is the better ideal experimental animal for providing a good experimental platform to study the pathogenesis of alcoholic fatty liver diseases.