ObjectiveTo evaluate the domestic ELISA kit for detection of mouse virus antibodies.MethodsDomestic and imported ELISA kits were choosen to detect five mouse virus antibodies , including lymphocytic choriomeningitis virus (LCMV), hepatitis virus (MHV), Sendai virus (SV), adenovirus (MAV), and parvovirus (MPV). The sensitivity, specificity, precision, stability, and reliability of the five kits were compared. ResultThere were significant differences in sensitivity of the same type domestic and imported kits, varying from at least 2 times (P<0.05) up to 16 times (P<0.01), and there were no cross reaction with the other four types of viruses. Precision test showed that the batch average coefficient of variation of the five kits was less than 10%. Stability test showed that the relative deviation of the five kits was less than 25%. 36 known mouse serum samples were tested with both the domestic and imported kits, respectively, and showed a coincidence rate of 100% for detection of LCMV, MHV, SV, and MPV. The domestic kit showed a coincidence rate of 86.1% in test for MAV, and that of the imported kit was 100%, with a very significant difference between them (P<0.01). ConclusionsExcept that the domestic MAV kit shows a lower sensitivity and credibility than that obtained with the imported kits, the domestic and imported LCMV, MHV, SV, MPV kits showed similar sensitivity, specificity, precision, stability and reliability.