Objective To establish commonly used experimental methods of Guizhou miniature pigs under the guidance of animal welfare and “3R” principle. MethodsUsing minimal irritative and less painful way to make intramuscular injection and intragastric gavage, blood collection, anesthesia, intravenous injection and euthanasia to Guizhou miniature pig. Results and ConclusionA set of experimental methods of Guizhou miniature pigs have been established in accordance with requirements of animal welfare and "3R" principle.