ObjectiveTo determine the genetic characteristics of PLCε knockout mice by polymorphic microsatellite DNA loci analysis．MethodsGenome DNA of 28 PLCε gene knockout mice were amplified by PCR using screening 15 microsatellite DNA loci,and population genetic diversity was identified by gene fragments．ResultsAmong 13 microsatellite DNA loci (D1Mit365,D3Mit51,D4Mit235,D6Mit102,D7Mit281,D8Mit113,D9Mit23,D10Mit180,D13Mit88,D16Mit145,D17Mit36,D18Mit94,D19Mit97),each locus of electrophoresis distance of DNA fragments in the 28 PLCε gene knockout mice kept consistent and presented monomorphism,indicating the genetic stability. However the two loci Dq (knock genotype) and Dy (wild-type) were used to discriminate 28 PLCε gene knockout mice by PCR amplification．Among them, 6 mice were of gene knock mice,7 mice were of wild-type mice,and 15 mice were of heterozygous type．ConclusionsThe method of microsatellite marker analysis can be used to monitor population genetic quality and accurately distinguish different genotypes of mice,providing a feasible method for the detection of genetic quality in mice.