The revised Directive for the protection of laboratory animals 2010/63/EU will be put into practice from January 2013. There are some articles amendment for animal welfare and 3Rs in new Directive comparative with its predecessor, which represent the new concepts for using animal testing and administration. The changes process of EU Directive and adjust for adapt to social status and scientific progress, which is worthy to reference for Chinese labanimal standardized management and assimilated to mainstream criterion. To understand the articles contents is useful to laboratory animal industries participate in the international competition, contrast research and provide specialized service in foreign trade.