ObjectiveIn order to improve the method of setting up the model of rat cryptorchidism mode, we explored a new method and also examined its stability. MethodTwenty-eight male Wistar rats were randomly divided into control (Ctrl) and model (Modl) group. Each rat was kept in a single cage. The Ctrl group can move freely during the experiment. The Modl group was tail-suspended for 3 weeks, and then reloaded for 8 weeks. Rats were sacrificed at the end of 3^rd week and 11^th week. The testis and epididymis were removed, weighted, then stained by Hematoxylin and Eosin Stainin, and examined under light microscopy. ResultsAfter 3-week tail-suspending, all rats’ testis slipped into enterocoelia. Compared with Ctrl Group, the weight of testis and epididymis were significantly decreased (P<0.01). After 8 weeks recovery, the weight of testis and epididymis did not increase to normal (P<0.01). With morphological examination, we found that after 3 weeks tail-suspending, seminiferous tubules were completely disorder and the spermatogonium were disappeared in the cauda epididymidis. The pathological changes were not improved(P<0.01)at the end of 11^th week. Conclusion3-week tail-suspending can simulate the pathological changes of cryptorchidism syndrome. It is much easier to be done than the traditional one. Moreover, this method has less injury on the rat. Therefore, it can be used to set up a new model of cryptorchidism syndrome.