Objective To establish proper model of human breast cancer in nude mice and study their biological features. Methods MDA-MB-231 and SK-BR-3 cells(ER-) were implanted into the left axilla of 10 nude mice. Tumor growth was observed, and the tumor-bearing mice were killed on day 42. The tumor masses were removed, and pathologically examined by the microscope. ResultsThe MDA-MB-231 tumor tubercles could be formed on day 5. The tumor-take rate was 90%(9/10),and average volume and weight of the tumor were (426.6±333.8)mm³ and (0.417±0.276) g reparatively. The SK-BR-3 tumor tubercles could be formed on day 11. The tumor-take rate was 80%(8/10),and average volume and weight of the tumor were (357.5±246.4)mm³ and (0.325±0.167) g reparatively. All of the specimens were infiltrating ductal carcinoma. Conclusion We succeeded in establishing two human breast cancer model in nude mice, which reserved certain biological features of human breast cancer, therefore it may be an available model for further research of breast cancer.