Abstract:Objective To study the application of cryopreservation of embryos of transgenic FVB mice expressing antibacterial peptides. Methods Superovulated FVB female mice and living for 4 - 6 weeks, 6 - 8 weeks, 8 - 10 weeks, respectively, were mated with heterozygote male mice. The 2-cell amphicytulas were collected and cryopreservted. One week later, the cryopreservted 2-cell amphicytulas were thawed and transplanted. Result From the 140 cryopreservted mouse embryos,98 viable embryos were obtained after thawing. Conclusion Embryo cryopreservation and embryo transfer technology can be used for transgenic mouse preservation.