Objective To investigate the effects of non-empirical factors including experimental operation,different raising style,the various Control Group,and addition of Vitamin C on physiological states of laboratory guinea pigs in GLP condition.Method Guinea pigs were divided into five groups with randomization by the computer software.The experiments were done in the GLP laboratory,where the temperature,humidity and noise were controlled according to the requirements.And the experiments were conducted according to the procedure recommended by the systemic anaphylaxis test in the Principle issued by the SFDA.Results In the allergizing period,the blank group and the dissolvent group had obvious distinction in the changes of body weight(P<0.05),which had no obvious distinction between the dissolvent group and the positive group during the three experimental stages(P>0.05).In comparison of the cage-raising style,the plastic box-raising style benefited the weight-increasing of pig guinea during the quarantine period(P<0.05).The body weight loss obviously in the group lack of Vitamin C compare to the control group(P<0.05).Conclusions Although the circumstance condition was greatly improved under the GLP condition,the non-empirical factors obviously influenced the physiological states of laboratory guinea pig.