The application of assisted reproductive technologies （ART） to nonhuman primates has created opportunities for improving reproductive management in breeding colonies, and for creation of new animal models by genetic modifi cation. One impediment to the application of ART in Saimiri spp. has been the lack of an effective gonadotropin preparation for ovarian stimulation. Pregnant mare serum gonadotropin （PMSG） is inexpensive and readily available, but its repeated use in rhesus monkeys has been associated with induction of a refractory state. We have compared PMSG to recombinant human follicle stimulating hormone （rhFSH） for controlled ovarian stimulation in Bolivian squirrel monkeys. Groups of mature squirrel monkeys received rhFSH （75 IU daily） or PMSG （250 IU twice daily） by subcutaneous injection for 4 d during the breeding season （November to January） or nonbreeding season （March to September）. Serum estradiol （E2） was measured daily. Follicular growth was monitored by abdominal ultrasound. During the breeding season, PMSG induced a higher E2 response than did rhFSH, with mean E2 levels being signifi cantly higher within 3 d of stimulation. Superior follicular development in PMSG animals was confi rmed by abdominal ultrasonography. During the nonbreeding season, PMSG elicited a similar increase in serum E2 levels despite the fact that basal serum E2 is typically low during the nonbreeding season. Repeated use of PMSG （ = 3 cycles of administration） produced no attenuation of the E2 response. We conclude that PMSG is highly effective for repeated cycles of controlled ovulation stimulation in the squirrel monkey.