Objective To prepare Cholecystokinin(CCK) transgenic mice and investigate the effect of CCK on food intake and metabolism in mice.Methods Transgenic mice were created by the method of microinjection.The genotype of transgenic lines was identified by PCR.The brain specific expression of the CCK gene was analyzed by Western Blotting.The plasma cholesterol and plasma triglyceride were detected and analyzed with a detection kit.Results Three founders of PDGF-CCK transgenic mice were established.Two of PDGF-CCK transgenic independent lines exhibited high-level brain-specific transgene expression compared with that of no-transgenic littermate controls by Western Blotting.The Body weight of the transgenic mice was lower than that of no-transgenic littermate controls,while the plasma cholesterol and plasma triglyceride were not detected obviously different from the controls.Conclusion High expression of CCK in the mouse brain can decrease the action of food intake and decrease body weight of the transgenic mice.