Objective To observe the changes on content of nitric oxide in serum and expression on epidermal growth factor receptor of gastric mucosa in cold stimulated rats. Methods Twenty SD rats were divided into control, model group at random, NO in serum and EGFR in gastric mucosa of rats were tested. Results The gastric mucosa were differently damaged in model group, and there were significant difference on content of nitric oxide in serum and expression of epidermal growth factor receptor in gastric mucosa between two groups. Conclusion Cold simulation may result in the gastric mucosa damage in rats, which also affect the content of nitric oxide in serum and expression of epidermal growth factor receptor in gastric mucosa of rats.