Objective To establish a RT-PCR method for detection of rabbit hemorrhagic disease virus (RHDV).Methods RHDV were purified from liver samples of rabbits died from RHDV infection,and total RNA was prepared.A 368 bp fragment was amplified by RT-PCR,then was cloned into pMEG-T vector.The recombinant plasmid was sequenced.Results The sequencing result of PCR products was homologous with the nucleotide sequence reported in the reference.The sensitivity assays indicated that 10~(-7) virus titer can be detected by PCR.Conclusion The established RT-PCR method for detection of RHDV is highly sensitive and specific.