Objective To investigate the changes of peripheral blood platelet count and function in the mouse model of idiopathic thrombocytopenic purpura(ITP) and to evaluate the therapeutic effect of a Chinese medicine Ningxuewan(NXW) on those changes.Methods The peripheral platelet count was conducted with an automatic hemocyte analyzer.CD41 and CD61 level was determined by flow cytometry. Results The amount of the peripheral blood platelets in ITP model mice was decreased after injection of antiplatelet serum(APS) and CD41 and CD61 were also decreased as compared with those before injection(P<0.001). NXW in high-dose increased the amount of platelets,and elevated the content of CD41 and CD61 to a normal level.Conclusion The platelet count and function are abnormal in mice in the episode of ITP.NXW can increase the amount of peripheral blood platelets and restore the aggregation function of platelets.