Objective To investigate the expression of heat shock protein (HSP) 27, 70 and 90 in Microtus fortis ovarian cancer. Methods Eight cases of spontaneous ovarian cancer and normal ovary were examined by histopathology and assessed for the expression of HSP27, HSP70 and HSP90 using immunohistochemical SP technique. Results All the 8 tumors were histopathologically diagnosed as serous cystadenocarcinoma. The positive-staining rates of HSP27, HSP70 and HSP90 in the cancers were 100%, 100% and 62%, respectively, while all were negative in normal ovary tissue. Conclusion The results demonstrate that HSP27 and HSP70 are overexpressed in the spontaneous ovarian cancers of Microtus Fortis, and the expression of HSP90 is lower. It is worthy of further study whether an animal model of ovarian cancer can be established from such tumor-bearing Microtus Fortis and to serve experimental studies on ovarian cancers.