Objective To establish animal models of intestinal flora regulation and intestinal flora imbalance prevention. Methods 1 The animal model of intestinal flora regulation:40 mice used in this experiment were randomly divided into 4 groups, each of 10 mice. Antibiotics were used for 3 days to create intestinal flora imbalance in mice. Then the mice of control group received saline and the mice in other 3 experimental groups received microecosystem regulator, fructose and traditional Chinese medicine, respectively, for 5 days for intestinal floras regulation. Feces samples of the mice were taken by aseptic manipulation and cultured for assessment of amount of enterobacteriaceae, bifidobacterium and lactobacillus floras growing in the intestine. 2 The model of intestinal flora imbalance prevention: 40 mice were randomly divided into 4 groups, each containing 10 mice. Along with the antibiotics, the mice in the 4 groups received saline, microecosystem regulator, fructose and traditional Chinese medicine, respectively, for 5 days. Samples of feces of the mice were taken after the completion of experiment and the amount of bacterial floras were assessed with the same procedure as in the first experiment. Results 1 The model of intestinal flora regulation:Compared with the control group using saline, all the 3 experimental groups showed that bifidobacterium, lactobacillus floras and intestinal bacillus were significantly increased after treatment with the microecosystem regulator, fructose and traditional Chinese medicine, respectively. 2 The model of intestinal flora imbalance prevention: Compared with the control group using normal saline, the bifidobacterium, lactobacillus floras and intestinal bacillus were significantly increased in the 3 experimental groups after treatment with microecosystem regulator, fructose and traditional Chinese medicine, respectively. Conclusion The microecosystem regulator, fructose and traditional Chinese medicine may excert effects to prevent the intestinal flora imbalance and increase the bifidobacterium, lactobacillus and intestinal bacillus floras in the intestine.