Objective To compare the three models of renal tubulointerstitial injury. Methods Gentamycin ip.(GM), unilateral ureteral obstruction(UUO), cyclosporine A(CsA) fed by oral were used to make the renal tubulointerstitial injury, then to detect urine biochemistry and blood biochemistry, and observe nephritic pathology. Results The nephridium indices of the models were all significantly higher in comparion with the control group(%p%<0^01). Urine protein and NAG enzyme of the GM model were significantly higher than the control on the 7th day, however, no significant difference was observed on the 15th day. Urine protein and NAG enzyme of the UUO model were significantly higher than the control on the 7th and 15th day, whereas urine protein of the CsA model was lower (7d %p%<0.05, 15d %p%<0.01). SCR and BUN of the three models were significantly higher than the control, whereas TP and ALB of the three models were significant lower than the control, There's no significant difference between the GM model and the UUO model on 15th day. Observation under light microscope showed inflammatory cell in interstitium, outspread of renal tubules, fibrosis in interstituium of the UUO model. The morphological changes were much slighter in the CsA model. Conclusion The three methods all could cause different level of renal tubulointerstitial injury. Fibrosis in interstitium of the UUO model was like the sign of clinical nepherosis. The UUO model was an ideal model for the study of the renal tubulointerstitial injury. The GM model seriously generating renal tubulointerstitial injury on the 7th day could be used for the acute model. However, the CsA model was not equal to the GM and the UUO models.;