Objective To find out the reles and obtain the reference data in the designs of barrier animal facilities.\ Methods The 24 charts of barrier animal facilities were analyzed in this study. Results The various facilities occurrence and their mean area were calculated in the barrier animal facilities. Many essential rooms there and their pecentage were offered:clean corridor 7.5%, dirty corridor 7.3%, first dressing room 1.6%, second dressing room 1.1%, bathroom 0.9%,air shower room 0.6%,buffer 4.2%, quarantine room 3.6%,breeding room or experimental room 39.8%, preparation room 5.0%, store room 3.3%, washing and sterile room 10.4%, machine room 10.8%, office 3.9%. The size of both contamination room and incinerator chamber varied with different conditions.Conclusion The data above can be useful in the designs of barrier animal facilities.