Objective To establish the technical data of transgene and cryopreservation. Method a)Five different genes were microinjected into ova, totaling 1864 1\|cell ova injected and 1283 2\|cell ova transferred. Finally we produced 20 transgenic mice. b)We got the optimum dosages of hormone for superovulation by five mouse strains injected with PMSG and HCG. Also we got average data of embryo superovulation of different strains. c)The embryo cryopreservation was produced by the normal method and glasses method. Results and Conclusion a)The integration rate of transgenic was 16.16% and the efficiency of gene transfer was 1.56%. b)The average superovulation number of different strains was 15.6 for C 57BL/6J (Japan) strain; 19.6 for C 57BL/6J; 21.4 for BDF1 (Japan) strain; 14.8 for C3H strain; 31 for KM strain. c)The ratio of the pups born after cryopreservation of mouse embryo was 30%-31%.