Objective\ To establish SPF and clean rabbits colony and manufacture base,and setup a technique to breeding and reproduce clean rabbits in the clean environment for the improvement of quality of experimental rabbits.Methods\ We use hysterectomy,tear the uterus in the isolator,let the pups anabiosis.The young were kept in germfree isolator,and were fed with artificial milk by hand,becoming germfree rabbits.5 kinds of microorganism were inoculated,one month later,the feces and serum of two rabbits were examined for microorganism and virus to confirm if SPF animal standard met.Results\ 52 specific pathogen\|free(SPF) rabbits have been obtained with the detection and confirmation of authorized institution.Conclusion\ We transfer baby rabbits to SPF and clean facility,in which they grow and reproduce well.It indicates that this technique is useful, repeatable and maneuverable.